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Soma Sekhar, M.
- Genotyping of Virulent Escherichia coli Obtained from Poultry and Poultry Farm Workers Using Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction
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Materials and Methods: Fecal swabs from different poultry species (n=150) and poultry farm workers (n=15) were analyzed for E. coli and screened for virulence genes (stx1, stx2, eaeA, and hlyA) by multiplex PCR. Virulent E. coli was serotyped based on their “O” antigen and then genotyped using ERIC-PCR.
Results: A total of 134 E. coli isolates (122/150 from poultry and 12/15 from farm workers) were recovered. Virulence genes were detected in a total of 12 isolates. Serological typing of the 12 virulent E. coli revealed nine different serotypes (O2, O49, O60, O63, O83, O101, O120, UT, and Rough). ERIC-PCR genotyping allowed discrimination of 12 virulent E. coli isolates into 11 ERIC-PCR genotypes. The numerical index of discrimination was 0.999.
Conclusion: Our findings provide information about the wide genetic diversity and discrimination of virulent E. coli in apparently healthy poultry and poultry farm workers of Andhra Pradesh (India) based on their genotype.
Authors
Affiliations
1 Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh, IN
2 Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, IN
3 Department of Animal Genetics and Breeding, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, IN
1 Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh, IN
2 Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, IN
3 Department of Animal Genetics and Breeding, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, IN
Source
Veterinary World, Vol 10, No 11 (2017), Pagination: 1292-1296Abstract
Aim: The aim of this study was to characterize virulent Escherichia coli isolated from different poultry species and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) genotyping.Materials and Methods: Fecal swabs from different poultry species (n=150) and poultry farm workers (n=15) were analyzed for E. coli and screened for virulence genes (stx1, stx2, eaeA, and hlyA) by multiplex PCR. Virulent E. coli was serotyped based on their “O” antigen and then genotyped using ERIC-PCR.
Results: A total of 134 E. coli isolates (122/150 from poultry and 12/15 from farm workers) were recovered. Virulence genes were detected in a total of 12 isolates. Serological typing of the 12 virulent E. coli revealed nine different serotypes (O2, O49, O60, O63, O83, O101, O120, UT, and Rough). ERIC-PCR genotyping allowed discrimination of 12 virulent E. coli isolates into 11 ERIC-PCR genotypes. The numerical index of discrimination was 0.999.
Conclusion: Our findings provide information about the wide genetic diversity and discrimination of virulent E. coli in apparently healthy poultry and poultry farm workers of Andhra Pradesh (India) based on their genotype.
Keywords
Escherichia coli, Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction, Genotype, Poultry, Serotype, Virulent.Full Text
- Virulence Gene Profiles of Arcobacter Species Isolated From Animals, Foods of Animal Origin, and Humans in Andhra Pradesh, India
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Materials and Methods: A total of 41 Arcobacter isolates (16 Arcobacter butzleri, 13 Arcobacter cryaerophilus, and 12 Arcobacter skirrowii) isolated from diverse sources such as fecal swabs of livestock (21), raw foods of animal origin (13), and human stool samples (7) were subjected to a set of six uniplex polymerase chain reaction assays targeting Arcobacter putative virulence genes (ciaB, pldA, tlyA, mviN, cadF, and cj1349).
Results: All the six virulence genes were detected among all the 16 A. butzleri isolates. Among the 13 A. cryaerophilus isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 61.5, 84.6, 76.9, 76.9, 61.5, and 61.5% of isolates, respectively. Among the 12 A. skirrowii isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 50.0, 91.6, 83.3, 66.6, 50, and 50% of isolates, respectively.
Conclusion: Putative virulence genes were detected in majority of the Arcobacter isolates examined. The results signify the potential of Arcobacter species as an emerging foodborne pathogen.
Authors
Affiliations
1 Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, IN
2 Department of Veterinary Microbiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, IN
3 Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, IN
1 Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, IN
2 Department of Veterinary Microbiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, IN
3 Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, IN
Source
Veterinary World, Vol 10, No 6 (2017), Pagination: 716-720Abstract
Aim: This study aimed to detect putative virulence genes in Arcobacter species of animal and human origin.Materials and Methods: A total of 41 Arcobacter isolates (16 Arcobacter butzleri, 13 Arcobacter cryaerophilus, and 12 Arcobacter skirrowii) isolated from diverse sources such as fecal swabs of livestock (21), raw foods of animal origin (13), and human stool samples (7) were subjected to a set of six uniplex polymerase chain reaction assays targeting Arcobacter putative virulence genes (ciaB, pldA, tlyA, mviN, cadF, and cj1349).
Results: All the six virulence genes were detected among all the 16 A. butzleri isolates. Among the 13 A. cryaerophilus isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 61.5, 84.6, 76.9, 76.9, 61.5, and 61.5% of isolates, respectively. Among the 12 A. skirrowii isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 50.0, 91.6, 83.3, 66.6, 50, and 50% of isolates, respectively.
Conclusion: Putative virulence genes were detected in majority of the Arcobacter isolates examined. The results signify the potential of Arcobacter species as an emerging foodborne pathogen.
Keywords
Arcobacter, Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii, Polymerase Chain Reaction, Virulence Genes.Full Text